BERBERINE INHIBITS CYTOSOLIC PHOSPHOLIPASE A2 AND PROTECTS AGAINST LPS-INDUCED LUNG INJURY AND LETHALITY INDEPENDENT OF THE α2-ADRENERGIC RECEPTOR IN MICE

• DOI: 10.1097/shk.0b013e318157ea14
• Publication Date: 2008-04-09T07:09:09Z
• Authors: Hao-qing Zhang; Hua-dong Wang; Da-xiang Lu; Ren-bin Qi; Yan-ping Wang; Yu-xia Yan; Yong-mei Fu
• Keywords: Lipopolysaccharides; 0301 basic medicine; Respiratory Distress Syndrome; Time Factors; Berberine; Neutrophils; Tumor Necrosis Factor-alpha; Phospholipases A2, Cytosolic; Yohimbine; Pulmonary Edema; Lung Injury; 3. Good health; Mice; 03 medical and health sciences; Receptors, Adrenergic, alpha-2; Animals; Phosphorylation; Bronchoalveolar Lavage Fluid

Acute lung injury is still a significant clinical problem having high mortality rate despite advances in antimicrobial therapy and supportive care made the past few years. Our previous study demonstrated that berberine (Ber) remarkably decreased attenuated mice challenged with LPS, but mechanism behind this remains unclear. Here, we report pretreatment Ber significantly reduced pulmonary edema, neutrophil infiltration, histopathological alterations; inhibited protein expression phosphorylation of cytosolic phospholipase A2; thromboxane A2 release induced by LPS. Yohimbine, an α2-adrenergic receptor antagonist, did not antagonize these actions Ber. Furthermore, TNF-α production which were enhanced yohimbine, combined yohimbine also improved survival subjected to cecal ligation puncture. Taken together, observations indicate attenuates LPS-induced inhibiting activation α2-adrenoceptor-independent manner. Berberine might provide effective therapeutic approach acute during sepsis.