BIPHASIC EFFECTS OF SELECTIVE INHIBITION OF TRANSFORMING GROWTH FACTOR β1 ACTIVIN RECEPTOR-LIKE KINASE ON LPS-INDUCED LUNG INJURY

• DOI: 10.1097/shk.0b013e3181aef736
• Publication Date: 2010-01-09T07:00:32Z
• Authors: Wei Wei; Ma Bing; Li Heng-yu; Jia Yitao; Lv Kaiyang; Wang Guangqing; Zhang Jianrong; Zhu Shihui; Tang Hongtai; Sheng Zhiyong; Xia Zhaofan
• Keywords: Hydroxyproline; Matrix metalloproteinase inhibitor

The present study was designed to find out whether SB431542, an inhibitor of transforming growth factor beta1 activin receptor-like kinase, could protect the lung from LPS-induced injury. Inflammatory injury model induced by intratracheal administration LPS. C57BL/6 mice were randomly divided into sham control group (S group), LPS stimulation (L + early SB431542 treatment (Ie and delayed (Id group). admitted intraperitoneally on days 1, 2, 3 in Ie group, whereas those Id received same dose 4, 5, 6. Pulmonary TNF-alpha IL- 1beta mRNA expressions tested. Pathological evaluations pulmonary alveolitis collagen deposition fibrosis performed 7 28, along with determination hydroxyproline, matrix metalloproteinase 9, tissue 1 day 28. As a result, resulted significant increases IL-1beta as well pathological scores for increased deposition, hydroxyproline content, decrease 9 activity. Those parameters further aggravated relieved significantly group. These data suggest that therapy inflammatory be harmful if during early-phase response. However, would prevent it initiated late.