Circulating Bacterial Membrane Vesicles Cause Sepsis in Rats

• DOI: 10.1097/shk.0b013e318250de5d
• Publication Date: 2012-03-07T00:55:12Z
• Authors: Bhairav Shah; Claretta J. Sullivan; Natalie E. Lonergan; Scott Stanley; Michael C. Soult; L.D. Britt
• Keywords: Male; Rats, Sprague-Dawley; 0303 health sciences; 03 medical and health sciences; Sepsis; Escherichia coli; Animals; Bacterial Outer Membrane Proteins; Rats; 3. Good health

Gram-negative bacteria remain the leading cause of sepsis, a disease that is consistently in top 10 causes death internationally. Curing bacteremia alone does not necessarily end process as other factors may inflammatory damage. Bacterial outer membrane vesicles (OMVs) are naturally produced blebs from gram-negative bacteria, which contain various proteins and lipopolysaccharide (LPS). We hypothesize these initiate an response independent parent bacteria. Outer were isolated cultures Escherichia coli, concentration LPS OMVs was measured. Adult male Sprague-Dawley rats separated into five treatment groups: OMV, 2xOMV, LPS, lactated Ringer's, sham. Our findings show infused elicit physiological, histological, molecular changes consistent with sepsis. Hyperdynamic heart rates mean arterial pressures observed well elevation proinflammatory cytokines tumor necrosis factor α interleukin 6. Downstream events such recruitment neutrophils tissues due to presentation vascular adhesion molecules also occur OMV-treated animals. Although soluble elicits stronger responses than did OMVs, latter exceeded those associated Ringer's infusion. These results indicate do response; however, further studies required better characterize temporal biomolecular interactions involved.