Intraoperative Blood Salvage During Liver Transplantation in Patients With Hepatocellular Carcinoma: Efficiency of Leukocyte Depletion Filters in the Removal of Tumor Cells

• DOI: 10.1097/tp.0b013e3181671f2e
• Publication Date: 2009-03-04T00:36:47Z
• Authors: Ting-Bo Liang; Dong-Lin Li; Liang Liang; Jun-Jian Li; Xue-Li Bai; Wei Yu; Wei-Lin Wang; Yan Shen; Min Zhang; Shu-Sen Zheng
• Keywords: Autotransfusion; Blood cell

Intraoperative blood salvage (IBS) reduces homologous transfusion in orthotopic liver transplantation (OLT), but may carry with it the risk of reinfusing tumor cells patients hepatocellular carcinoma (HCC). The use leukocyte depletion filters (LDFs) for removal is rarely reported clinical OLT. aims this study were to evaluate frequency cell contamination surgical field during OLT HCC recipients and investigate efficiency additional LDFs eliminating from IBS.Thirty-two preoperatively elevated serum alpha-fetoprotein (AFP) underwent was collected processed by an autotransfusion device (Cell Saver 5), followed 2 consecutive LDF filtrations. IBS samples filtered determined using a nested RT-PCR technique detect AFP mRNA.The shed 20 (62.5%) 32 contaminated 15 them remained positive after Cell processing. Patients within Milan or UCSF criteria less likely have more be removed these as compared other (P<0.01). After filtration through LDF, most cases (13/15) became negative except those ruptured tumors (P<0.05).Our results suggest that can efficiently remove could markedly reduce reintroduction nonruptured tumors.