Detection of hepatitis B virus genotypes in a group of hepatitis B virus-infected patients in central and northern Sri Lanka

• DOI: 10.1099/acmi.0.000838.v3
• Publication Date: 2024-10-03T16:19:02Z
• Authors: T. T. Pattiyakumbura; K. G. K. Malkanthi; W. K. H. Dheerase...; A. Manamperi; M. A. R. V. Muthu...
• Keywords: Sri lanka

Introduction. Hepatitis B infection causes a spectrum of clinical diseases varying from asymptomatic to severe or fulminant acute hepatitis, chronic liver disease, cirrhosis and hepatocellular carcinoma. virus (HBV) genotypes appear influence transmission dynamics, outcomes responses antiviral therapy. However, hepatitis genotyping has been poorly investigated in Sri Lanka. This study intended determine group HBV-infected people central northern Methodology. The was laboratory-based descriptive cross-sectional study. Initial detection HBV DNA 100 EDTA blood samples done by using commercially validated quantitative real-time PCR kit. performed in-house conventional semi-nested multiplex genotype-specific primers (for A–F). serological profile determined ELISA/chemiluminescence immunoassay. results were evaluated for genotype prevalence, viral load association e antigen (HBeAg) expression the population. Results conclusion. detected that C ( n =38) is most prevalent infections with multiple =52, 52%) commoner than mono-genotype =23, 23%) infections. In total, 25% patients had no detectable among A–F. mean single 3.28 log 10 copies ml –1 4.18 before treatment. Statistical significance not loads HBeAg these two groups. future, may be effectively treated managed according infected genotype.