<xhtml:span xmlns:xhtml="http://www.w3.org/1999/xhtml" xml:lang="en">Effect of Antibiotic Administration on&#160;Blastocystis&#160;Persistence and Gut Microbiome-Metabolome Dynamics in an Irritable Bowel Syndrome Longitudinal Case Study </xhtml:span>

• DOI: 10.1099/acmi.0.000926.v2
• Publication Date: 2025-01-30T17:31:03Z
• Authors: Jamie M. Newton; William JS Edwards; Gary S. Thompson; Eleni Gentekaki; Anastasios D. Tsa...

Background: Blastocystis, the most prevalent microbial eukaryote in humans, has a global distribution. Studies have linked its presence with distinct gut microbiome and metabolome profiles compared to those where organism is absent. However, interplay of antibiotics administration, Blastocystis surrounding remains understudied. This case study aimed explore antibiotic consumption subsequent changes an individual diagnosed irritable bowel syndrome (IBS). Methods: Stool samples from IBS patient, collected at 12 time points, were tested for using RT-PCR targeting SSUrRNA gene, followed by sequencing positive samples. Illumina determined composition, while one-dimensional proton NMR spectroscopy was used analyse composition. Statistical analyses conducted identify relationships between consumption, bacterial diversity, presence. Results: Antibiotics significantly impacted microbiome, diversity declining early course, then recovering later post-course. detected early, late, post-course but not detectable mid-course, coinciding decline diversity. Significant differences observed Blastocystis-positive Blastocystis-negative composition changing before, after course mid-course. Metabolite groups, including short-chain fatty acids, amino succinate, exhibited throughout indicating that metabolite affected consumption. Discussion/Conclusion: While did impact colonisation, they cause mid-course The also revealed significant alterations important metabolites such as SCFAs acids altered underscores complex interactions antibiotics, microbiota, metabolites, highlighting resilience ecosystem.