Deferribacter autotrophicus sp. nov., an iron(III)-reducing bacterium from a deep-sea hydrothermal vent

• DOI: 10.1099/ijs.0.006767-0
• Publication Date: 2009-06-05T19:49:30Z
• Authors: G. B. Slobodkina; T. V. Kolganova; N. A. Chernyh; J. Querellou; E. A. Bonch-Osmol...; A. I. Slobodkin
• Keywords: Strain (injury); Electron acceptor

A thermophilic, anaerobic, chemolithoautotrophic bacterium (designated strain SL50T) was isolated from a hydrothermal sample collected at the Mid-Atlantic Ridge deepest of known World ocean fields, Ashadze field (1 ° 58′ 21″ N 4 51′ 47″ W) depth 4100 m. Cells SL50T were motile, straight to bent rods with one polar flagellum, 0.5–0.6 μm in width and 3.0–3.5 length. The temperature range for growth 25–75 °C, an optimum 60 °C. pH 5.0–7.5, 6.5. Growth observed NaCl concentrations ranging 1.0 6.0 % (w/v) 2.5 (w/v). generation time under optimal conditions min. Strain used molecular hydrogen, acetate, lactate, succinate, pyruvate complex proteinaceous compounds as electron donors, Fe(III), Mn(IV), nitrate or elemental sulfur acceptors. G+C content DNA 28.7 mol%. 16S rRNA gene sequence analysis revealed that closest relative Deferribacter abyssi JRT (95.5 similarity). On basis its physiological properties phylogenetic analyses, isolate is considered represent novel species, which name autotrophicus sp. nov. proposed. type (=DSM 21529T=VKPM B-10097T). first described deep-sea capable using hydrogen donor ferric iron acceptor CO2 carbon source.