Nocardioides hungaricus sp. nov., isolated from a drinking water supply system

• DOI: 10.1099/ijs.0.022939-0
• Publication Date: 2010-04-10T01:11:23Z
• Authors: Erika M. Tóth; Zsuzsa Kéki; Judit Makk; Zalán G. Homonnay; Károly Márialigeti; Peter Schumann
• Keywords: DNA, Bacterial; Base Composition; Hungary; 0303 health sciences; Fatty Acids; Molecular Sequence Data; Vitamin K 2; Sequence Analysis, DNA; Diaminopimelic Acid; DNA, Ribosomal; 6. Clean water; Bacterial Typing Techniques; 03 medical and health sciences; Water Supply; RNA, Ribosomal, 16S; Actinomycetales; Cluster Analysis; Water Microbiology; Phylogeny

Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4T, Nocardioides aquiterrae GW-9T, Nocardioides sediminis MSL-01T and N. hankookensis DS-30T. The peptidoglycan based on ll-2,6-diaminopimelic acid, the major menaquinone MK-8(H4), the cellular fatty acid profile with iso-C16 : 0 and anteiso-C17 : 0 as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12T) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1 % 16S rRNA gene sequence similarity between strain 1RaM5-12T and its closest phylogenetic neighbour, N. pyridinolyticus OS4T, a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12T (=DSM 21673T =NCAIM 02330T).