Devosia psychrophila sp. nov. and Devosia glacialis sp. nov., from alpine glacier cryoconite, and an emended description of the genus Devosia

• DOI: 10.1099/ijs.0.023937-0
• Publication Date: 2011-05-07T05:26:49Z
• Authors: De-Chao Zhang; Mersiha Redzic; Hong-Can Liu; Yu-Guang Zhou; Franz Schinner; Rosa Margesin
• Keywords: DNA, Bacterial; Base Composition; Hyphomicrobiaceae; 0303 health sciences; Ubiquinone; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Sequence Analysis, DNA; DNA, Ribosomal; Bacterial Typing Techniques; Cold Temperature; 03 medical and health sciences; RNA, Ribosomal, 16S; Cluster Analysis; Ice Cover; Phospholipids; Phylogeny

Two psychrophilic strains, Cr7-05(T) and Cr4-44(T), isolated from alpine glacier cryoconite, were characterized by using a polyphasic approach. Both strains psychrophilic, showing good growth over temperature range of 1-20 °C. The chemotaxonomic characteristics these isolates included the presence C(18:1)ω7c summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c) as major cellular fatty acids, Q-10 predominant ubiquinone diphosphatidylglycerol, phosphatidylglycerol unknown glycolipids polar lipids. DNA G+C contents Cr4-44(T) 61.4 63.6 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that two belong to genus Devosia. sequence similarity between was 98.6%, but DNA-DNA hybridization 54% relatedness. Strains exhibited 94.7-97.2 94.9-96.9%, respectively, type recognized Devosia species. On basis phenotypic characteristics, phylogenetic relatedness data, represent novel species within Devosia, for which names psychrophila sp. nov. (type strain =DSM 22950(T) =CGMCC 1.10210(T) =CIP 110130(T)) glacialis 1.10691(T) =LMG 26051(T)) are proposed. An emended description is also provided.