Rothia endophytica sp. nov., an actinobacterium isolated from Dysophylla stellata (Lour.) Benth

• DOI: 10.1099/ijs.0.052522-0
• Publication Date: 2013-05-25T05:42:29Z
• Authors: Zi-Jun Xiong; Jin-Li Zhang; Dao-Feng Zhang; Zhi-Li Zhou; Min-Jiao Liu; Wen-Yong Zhu; Li-Xing Zhao; Li-Hua Xu; Wen-Jun Li
• Keywords: Strain (injury)

A novel endophytic actinobacterium, designated strain YIM 67072 T , was isolated from healthy roots of Dysophylla stellata (Lour.) Benth. Cells this aerobic, cream–yellow-coloured occurred singly, in pairs or tetrads, were Gram-stain-positive and ovoid- to spherical-shaped. Strain grew at 4–45 °C, pH 5.0–10.0 the presence 0–7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that belonged genus Rothia . The isolate contained MK-7 as major component quinone system. peptidoglycan type A3α. polar lipid profile consisted predominantly diphosphatidylglycerol glycolipids. fatty acids anteiso-C 15 : 0 iso-C 17 16 DNA G+C content 53.2 mol%. However, differed its closest relatives nasimurium CCUG 35957 (98.5 sequence similarity), amarae JCM 11375 (97.6 %) terrae L-143 (97.3 many phenotypic characteristics. Moreover, levels DNA–DNA relatedness between three above-mentioned strains 28.7±1.3 %, 36.5±1.2 46.8±1.5 respectively. Based comparative physiological chemotaxonomic data, represents a species for which name endophytica sp. nov. is proposed. ( = DSM 26247 18541 ).