Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti

• DOI: 10.1099/ijs.0.2008/001032-0
• Publication Date: 2008-12-05T18:05:16Z
• Authors: M. Kraatz; D. Taras
• Keywords: Veillonella; Coccus (insect)

A bacterium, designated strain lac18T, was isolated in pure culture from the mucosal jejunum of a healthy pig, using medium selective for anaerobic lactic acid bacteria and containing porcine gastric mucin as main carbon nitrogen source. Cells this were coccus-shaped, arranged singly or pairs Gram-stain-negative, oxidase-negative, non-spore-forming, microaerotolerant. An analysis based on 16S rRNA gene sequences indicated that lac18T should be assigned to genus Veillonella, class Clostridia, phylum Firmicutes. dnaK sequence-based phylogenetic analyses both most closely related species Veillonella ratti ATCC 17746T (similarities 96.6 84.5 %, respectively) criceti 17747T 83.4 respectively). The results DNA–DNA hybridizations between these type genus, parvula DSM 2008T, confirmed genotypic distinctness novel isolate. Data phenotypic studies also served differentiate strains. Therefore represents which name magna sp. nov. is proposed. (=CCUG 55454T=CIP 109767T=DSM 19857T=JCM 15053T).