Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)

• DOI: 10.1099/ijsem.0.000998
• Publication Date: 2016-03-01T00:38:43Z
• Authors: Holger C. Scholz; Sandra Revilla-Fe...; Sascha Al Dahouk; Jens A. Hammerl; Michel S. Zygmunt; Axel Cloeckaert; Mark Koylass; Adrian M. Whatmore; Jochen Blom; Gilles Vergnaud; Angela Witte; Karin Aistleitner; Erwin Hofer
• Keywords: DNA, Bacterial; 0301 basic medicine; 106022 Mikrobiologie; Base Composition; Foxes; Sequence Analysis, DNA; Brucella; Bacterial Typing Techniques; 03 medical and health sciences; Austria; 106022 Microbiology; Animals; Lymph Nodes; Bacteriophage Typing; Phylogeny

Two slow-growing, Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains F60T and F965), isolated in Austria from mandibular lymph nodes of two red foxes (Vulpes vulpes), were subjected to a polyphasic taxonomic analysis. In recent study, both isolates assigned the genus Brucella but could not be attributed any existing species. Hence, we have analysed strains further detail determine their exact position genetic relatedness other members Brucella. The genome sizes F965 3 236 779 237 765 bp, respectively. Each consisted chromosomes, with DNA G+C content 57.2 %. A genome-to-genome distance >80 %, an average nucleotide identity (ANI) 97 % amino acid (AAI) 98 compared type species melitensis confirmed affiliation genus. Remarkably, 5 entire information was non-Brucella origin, including as-yet uncharacterized bacteriophages insertion sequences as well ABC transporters genes metabolic function various soil-living bacteria. Core-genome-based phylogenetic reconstructions placed novel separated all hitherto-described Brucella, forming long-branched sister clade classical summary, based on phenotypic molecular data, conclude that are for which name vulpis sp. nov. is proposed, strain ( = BCCN 09-2T DSM 101715T).