Cryobacterium ruanii sp. nov. and Cryobacterium breve sp. nov., isolated from glaciers

• DOI: 10.1099/ijsem.0.003994
• Publication Date: 2020-02-25T18:13:25Z
• Authors: Qing Liu; Ji-Hui Tian; Hong-Can Liu; Yu-Guang Zhou; Yu-Hua Xin
• Keywords: Phosphatidylglycerol; Strain (injury)

Strains Sr36 T and TMT4-23 were isolated from No. 1 glacier in Xinjiang Uygur Autonomous Region Toumingmengke Gansu Province, PR China, respectively. They Gram-stain-positive rod-shaped micro-organisms. The optimum growth temperature of the two strains was 10–14 °C. Phylogenetic analysis showed that related to members genus Cryobacterium . average nucleotide identity (ANI) digital DNA–DNA hybridization (dDDH) values between strain its close relatives luteum Hh15 , aureum Hh31 levicorallinum Hh34 flavum Hh8 81.16–87.24 28.0–32.5 %, ANI dDDH relative psychrotolerans 0549 81.16 22.3 %. polar lipids diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid three lipids. glycolipid, phospholipid six major fatty acids anteiso-C 15 : 0 iso-C 16 17 Both contained 2,4-diaminobutyric acid their predominant menaquinone MK-10. On basis phenotypic, phylogenetic genotypic data, novel species ruanii sp. nov. (type = =CGMCC 1.9275 =NBRC 113797 ) breve =TMT4-23 1.9556 113800 are proposed.