Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk

• DOI: 10.1099/ijsem.0.004125
• Publication Date: 2020-03-30T15:19:52Z
• Authors: Alexander Flegler; Katharina Runzheimer; Vanessa Kombeitz; Anna Tatjana Mänz; David Heidler von...; Lara Etzbach; Andreas Schieber; Georg Hölzl; Bruno Hüttel; Christian Woehle; André Lipski
• Keywords: Arthrobacter; Cow milk

A pink-coloured bacterium (strain KR32T) was isolated from cheese and assigned to the 'Arthrobacter agilis group'. Members of 'pink Arthrobacter group' form a stable clade (100 % bootstrap value) contain species agilis, ruber echini, which share ≥99.0 16S rRNA gene sequence similarity. Isolate KR32T showed highest similarity (99.9 %) A. DSM 20550T. Additional multilocus comparison confirmed assignment strain Average nucleotide identity digital DNA-DNA hybridization values between isolate 20550T were 82.85 26.30 %, respectively. The G+C content genomic DNA 69.14 mol%. Chemotaxonomic analysis determined anteiso-C15 : 0 as predominant fatty acid MK-9(H2) menaquinone. Polar lipids diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol monoacyldimannosyl-monoacylglycerol. peptidoglycan type A3α. carotenoid bacterioruberin detected major pigment. At 10 °C, grew with increased concentrations production unsaturated acids. Strain Gram-stain-positive, catalase-positive, oxidase-positive coccus-shaped optimal growth at 27-30 °C pH 8. results phylogenetic phenotypic analyses enabled differentiation other closely related Therefore, represents novel for name bussei sp. nov. is proposed. (=DSM 109896T=LMG 31480T=NCCB 100733T).