Chitinophaga extrema sp. nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest

• DOI: 10.1099/ijsem.0.004539
• Publication Date: 2020-11-04T17:22:56Z
• Authors: Calvin Bok Sun Goh; Li Wen Wong; Sivachandran Pari...; Heera Rajandas; Stella Loke; Lawrence Croft; Catherine Mary Yule; Pooria Pasbakhsh; Sui Mae Lee; Joash Ban Lee Tan
• Keywords: Strain (injury); Soil microbiology; Swamp

A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1T was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at base of a Koompassia malaccensis tree in tropical peat swamp forest northern regions state Selangor, Malaysia (3° 39' 04.7' N 101° 17' 43.7'' E). Phylogenetic analyses based full 16S rRNA sequence revealed that belongs to genus Chitinophaga with greatest similarity terrae KP01T (97.65 %), jiangningensis DSM27406T (97.58 dinghuensis DHOC24T (97.17 %). The major fatty acids (>10 %) are iso-C15 : 0, C16 1 ω5c iso-C17 0 3-OH while predominant respiratory quinone is menaquinone-7. Strain has complete genome size 8.03 Mb, G+C content 48.5 mol%. DNA-DNA hybridization (DDH) score between C. 15.9 %, silico DDH values against were 20.0 19.10% respectively. Concurrently, Average Nucleotide Identity (ANI) scores all three reference strains 73.2 %. Based phenotypic, chemotaxonomic, phylogenetic consensus, represents novel species Chitinophaga, for which name extrema sp. nov. proposed (=DSM 108835T=JCM 33276T).