Pseudomonas germanica sp. nov., isolated from Iris germanica rhizomes

• DOI: 10.1099/ijsem.0.005268
• Publication Date: 2022-03-21T18:17:21Z
• Authors: Kostadin Evgeniev...; David Miñana Galbis; Julia Gallego; Annabel Serpico; Montserrat Bosch; Teresa Altabella; Albert Ferrer
• Keywords: Pseudomonas fluorescens; rpoB

Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-negative, aerobic, catalase- and oxidase-positive gammaproteobacterial strain, referred to as FIT28 T , was isolated. shows vigorous growth on nutrient rich media within the temperature range 4–35 °C, with optimal at 28 wide pH tolerance 5 11, salt up 6 % (w/v) NaCl. Colonies are white-yellow quickly become mucoid. The results analysis 16S rRNA gene sequence placed strain genus Pseudomonas multilocus (MLSA) using rRNA, rpoB gyrB rpoD concatenated sequences revealed that closest relatives zeae OE48.2 ' crudilactis UCMA 17988, tensinigenes ZA5.3 helmanticensis OHA11 baetica a390 iridis P42 atagonensis PS14 koreensis Ps 9-14 subgroup fluorescens lineage. genome size is about 6.7 Mb 59.09 mol% DNA G+C content. Average nucleotide identity (ANI) digital DNA–DNA hybridization (dDDH) values calculated genomic closely related P. 95.23 63.4 %, respectively. Biochemical, metabolic chemotaxonomic studies further support our proposal sp. nov., should be considered novel species . Hence, type (=LMG 32353 =DSM 112698 ) has been deposited in public cell-type culture centres.