Molecular epidemiology of beta-lactamase producing nosocomial Gram-negative pathogens from North and South Indian hospitals

• DOI: 10.1099/jmm.0.000513
• Publication Date: 2017-07-03T14:14:36Z
• Authors: Surbhi Khurana; Purva Mathur; Arti Kapil; Chithra Valsan; Bijayini Behera
• Keywords: Colistin; Beta-lactamase

Purpose. Resistant Gram-negative bacterial (GNB) infections, apart from tremendously escalating the cost of treatment, are a cause for substantial morbidity and mortality among hospitalized patients. Such bacteria rapidly acquiring resistance to many antimicrobial agents, especially beta-lactams which most frequently prescribed antimicrobials in hospital community patient care settings, now also colistin; last-line drug treat infections with such bacteria. The greatest threat treatment is production metallo beta-lactamases, plasmid-mediated serine carbapenemases. Methodology. We conducted two-year study observe pattern beta-lactamase enzyme (extended spectrum beta-lactamases (ESBLs), AmpC carbapenemases) nosocomial GNB isolated intensive units (ICUs) North South Indian hospitals. A total 761 non-duplicate were included (554; 73 %) India (207; 27 %). All strains subjected Clinical Laboratory Standards Institute (CLSI) recommended screening tests detection production, followed by polymerase chain reaction (PCR)-based clinically important genes mediating resistant phenotypes these isolates. Results. Out GNB, Acinetobacter spp., Klebsiella Pseudomonas Enterobacter spp. others 27, 23 , 21 17 5 7 % respectively. high prevalence ESBL was found across all genera strains. carbapenem higher than GNB. level comparatively lower both Conclusion. Beta-lactamases showed tremendous variation geographic distribution. Thus, their characterization clinical-epidemiological, laboratory infection control point view. Knowledge this epidemiology can predict empiric treatment.