Prosthetic joint infections present diverse and unique microbial communities using combined whole-genome shotgun sequencing and culturing methods

• DOI: 10.1099/jmm.0.001068
• Publication Date: 2019-08-28T16:14:35Z
• Authors: Abigail A. Weaver; Nur A. Hasan; Mark Klaassen; Hiren Karathia; Rita R. Colwell; Joshua D. Shrout
• Keywords: Shotgun; Microbial genetics

Introduction. Prosthetic joint infections (PJIs) are challenging to treat therapeutically because the infectious agents often resistant antibiotics and capable of abundant growth in surface-attached biofilms. Though infection rates low, ca. 1-2 %, overall increase sheer number replacement surgeries results an patients at risk.Aims. This study investigates consensus microbial species comprising PJI ecology, which is currently lacking.Methodology. In this study, populations from seven were analysed using combined culturing whole-genome shotgun sequencing (WGSS) establish population profiles compare WGSS culture methods for detection identification microbiome.Results. detected strains when did not, notably dormant, culture-resistant rare microbes. The CosmosID algorithm was used predict micro-organisms present discriminate contaminants. However, indicated presence microbes falling below threshold. these instances, cultured believed be minor species. two strategies build a profile.Conclusions. Variability between among PJIs showed that most distinct unique. Comparative analysis revealed form clusters related to, but separate from, vaginal, skin gut microbiomes. Fungi protists by WGSS, role fungi just beginning understood it unknown. These their novel strain-specific interactions remain determined current clinical tests.