Two cases of failed ceftriaxone treatment in pharyngeal gonorrhoea verified by molecular microbiological methods

• DOI: 10.1099/jmm.0.007641-0
• Publication Date: 2009-04-15T17:38:13Z
• Authors: John Tapsall; Phillip Read; Christopher Carmody; Christopher Bourne; Sanghamitra Ray; Athena Limnios; Theo Sloots; David Whiley
• Keywords: Adult; Male; 0301 basic medicine; Chlamydia trachomatis; Polymerase Chain Reaction; 060501 Bacteriology; Gonorrhea; 03 medical and health sciences; C1; Humans; Treatment Failure; gonorrhoea; 060502 Infectious Agents; 060503 Microbial Genetics; Ceftriaxone; ceftrizxone; Homosexuality; Pharyngeal Diseases; Neisseria gonorrhoeae; Anti-Bacterial Agents; 3. Good health; Treatment; 920203 Diagnostic Methods; Female

Diagnostic, genotypic and antibiotic-resistance determinants ofNeisseria gonorrhoeaewere analysed by molecular methods to verify the failure of ceftriaxone treatment in two cases of pharyngeal gonorrhoea. Monoplex assays were needed to define competitive inhibition of a positiveChlamydiaPCR in a duplex assay. DifferentpenAchanges were detected in theN. gonorrhoeaeisolated from the two cases. These were associated with raised ceftriaxone MICs of 0.03 and 0.016 mg l−1, which may have contributed to the treatment failures in these cases.