Corynebacterium diphtheriae as an emerging pathogen in nephrostomy catheter-related infection: evaluation of traits associated with bacterial virulence

• DOI: 10.1099/jmm.0.012161-0
• Publication Date: 2009-10-09T18:39:15Z
• Authors: Débora L. R. Gomes; Carlos A. S. Martins; Lúcia M. D. Faria; Louisy S. Santos; Cintia S. Santos; Priscila S. Sabba...; Mônica C. Souza; Gabriela B. Alves; Ana C. P. Rosa; Prescilla E. Nagao; Gabriela A. Pereira; Raphael Hirata; Ana L. Mattos-Gua...
• Keywords: Corynebacterium diphtheriae

Corynebacterium diphtheriae still represents a global medical challenge, particularly due to the significant number of individuals susceptible diphtheria and emergence non-toxigenic strains as causative agents invasive infections. In this study, we characterized clinical microbiological features what believe be first case C. infection percutaneous nephrostomy catheter insertion site in an elderly patient with fatal bladder cancer. Moreover, demonstrated potential role adherence, biofilm formation fibrin deposition traits from catheter-related infection. Non-toxigenic isolated purulent discharge (named strain BR-CAT5003748) was identified by API Coryne system (code 1 010 324) multiplex PCR for detection dtxR tox genes. Strain BR-CAT5003748 showed resistance oxacillin, ceftazidime ciprofloxacin. experiments performed vitro, isolate classified moderately hydrophobic adherent polystyrene surfaces. Glass provided more effective surface than polystyrene. Micro-organisms adhered (>1.5 x 10(6) c.f.u.) multiplied on surfaces polyurethane catheters. Microcolony (a hallmark formation) amorphous accretions were observed scanning electron microscopy both external luminal yielded simultaneous expression localized adherence-like aggregative-like (LAL/AAL) adherence patterns HEp-2 cells. Interestingly, coagulase tube test resulted thin layer embedded rabbit plasma strain. conclusion, should recognized cause infections at-risk populations such cancer patients. LAL/AAL may associated virulence that enable effectively produce biofilms Biofilm could have contributed persistence at infected obstruction catheter.