Development of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service

• DOI: 10.1099/jmm.0.05213-0
• Publication Date: 2003-07-17T04:01:24Z
• Authors: Kathryn A. Harris; John C. Hartley
• Keywords: Clinical Microbiology; Microbiological culture; Microbiological Techniques

The aim of the present study was to develop a broad-range PCR based on bacterial 16S rDNA for use in routine diagnostic clinical microbiology service. optimization and validation assay specimens from normally sterile sites is described, preliminary results are reported diagnosis infection 382 paediatric over 2-year period. These compared those obtained by standard culture techniques show increased when both used together; provided sole evidence pathogenic 71 cases. Key stages development potential pitfalls technique highlighted improvement offers setting discussed.