Resistotype of Helicobacter pylori isolates: the impact on eradication outcome

• DOI: 10.1099/jmm.0.069781-0
• Publication Date: 2014-04-22T16:20:43Z
• Authors: Hanafiah Alfizah; Ahmad Norazah; Razlan Hamizah; Mohamed Ramelah
• Keywords: Etest; 23S ribosomal RNA; Nonsense mutation

Antibiotic resistance is increasing worldwide, and it has been regarded as the main factor reducing efficacy of Helicobacter pylori therapy. The aim this study was to determine phenotype genotype antibiotic-resistant strains H. in Malaysian population evaluate impact antibiotic eradication outcome. One hundred sixty-one isolates were analysed study. Metronidazole, clarithromycin, fluoroquinolone, amoxicillin tetracycline susceptibilities determined by Etest. PCR followed DNA sequencing carried out mutations. medical records patients infected with resistant reviewed Metronidazole encountered 36.6 % isolates, whereas clarithromycin fluoroquinolone observed 1.2 1.9 respectively. All tested susceptible tetracycline. Frameshift nonsense mutations rdxA frxA genes resulting stop codons contributed metronidazole resistance, which leads reduced efficacy. A2142G A2143G 23S rRNA identified causing failure Mutation at either codon 87 or 91 gyrA gene fluoroquinolone-resistant strains. However, effect could not be assessed. This showed that frameshift point affected