Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods

• DOI: 10.1099/jmm.0.46218-0
• Publication Date: 2005-12-30T21:02:36Z
• Authors: Philip Suffys; Adalgiza da Silva...; Adeilton Brandão; Bart Vanderborght; Wouter Mijs; Geert Jannes; Fernanda C. Q. Mello; Heloisa da Silvei...; Leila de Souza Fo...; Rosângela Siqueir...; Sylvia Cardoso Leão; Maria Helena Fére...
• Keywords: Nontuberculous Mycobacteria

Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates a woman with unknown HIV status, had been identified as belonging to Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such PCR-restriction enzyme (PRA) hsp65 gene, commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing 16S-23S internal transcribed spacer (ITS), presence lentiflavum was also demonstrated. At time detection, cases were infected M. avium, suggesting underestimation infection co-infection different species.