FSY1, a horizontally transferred gene in the Saccharomyces cerevisiae EC1118 wine yeast strain, encodes a high-affinity fructose/H+ symporter

• DOI: 10.1099/mic.0.041673-0
• Publication Date: 2010-08-13T00:44:07Z
• Authors: Virginie Galeote; Maïté Novo; Madalena Salema-Oom; Christian Brion; Elisabete Valério; Paula Gonçalves; Sylvie Dequin
• Keywords: Hexose

Transport of glucose and fructose in the yeast Saccharomyces cerevisiae plays a crucial role controlling rate wine fermentation. In S. cerevisiae, hexoses are transported by facilitated diffusion via hexose carriers (Hxt), which prefer to fructose. However, utilization is critically important at end Here, we report characterization transporter recently identified sequencing genome commercial strain EC1118 found many other yeasts. This designated Fsy1p because its homology with pastorianus fructose/H(+) symporter Fsy1p. A obtained transformation V5 hxt1-7Δ mutant FSY1 grew well on fructose, but much lesser extent as sole carbon source. Sugar uptake symport experiments showed that encodes proton-coupled high affinity for (K(m) 0.24±0.04mM). Using real-time RT-PCR, also investigated expression pattern growing various sources. was repressed concentrations or highly expressed ethanol The characteristics this indicate acquisition could confer significant advantage during fermentation process. good example new function horizontal gene transfer.