Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro

• DOI: 10.1099/mic.0.2006/000711-0
• Publication Date: 2007-01-27T01:09:22Z
• Authors: Catherine M. Byrne; Marguerite. Clyne; Billy. Bourke
• Keywords: Internalization; Intestinal mucosa

Campylobacter jejuni is a common cause of serious diarrhoeal disease in humans, contrast to the avian population, where exposure results prolonged colonization at high density without disease. Colonized poultry present significant source infection humans worldwide. The aim this work was compare interaction with primary intestinal cells from and identify factors that account for divergent outcome following exposure. A cell model developed using grown human chicken biopsies. cultured were infected number strains Campylobacter. Invasion by C. influence mucus on internalization studied fluorescence microscopy gentamicin protection assays. invaded microtubule-, microfilament- caveolin-dependent manner. Entry into also occurred. Chicken mucus, but not origin, significantly reduced cells. Avian appears inhibit interacting epithelial surfaces.