Strain-specific proteome responses of Pseudomonas aeruginosa to biofilm-associated growth and to calcium

• DOI: 10.1099/mic.0.2007/010371-0
• Publication Date: 2007-11-01T19:33:29Z
• Authors: Marianna A. Patra...; Svetlana A. Sarki...; Michael J. Franklin
• Keywords: Proteome; Strain (injury); Pyocyanin

Pseudomonas aeruginosa is an opportunistic pathogen that forms biofilms on mucous plugs in the lungs of cystic fibrosis (CF) patients, resulting chronic infections. Pulmonary P. isolates often display a mucoid (alginate-producing) phenotype, whereas non-mucoid strains are generally associated with acute We characterized cytosolic proteomes biofilm-associated and planktonic CF pulmonary isolate, FRD1, strain, PAO1. Since Ca2+ metabolism altered fluids, we also analysed effect proteome responses these strains. Both abundances 40–60 % their proteins response to biofilm growth and/or [Ca2+]. Differentially expressed clustered into 12 groups, based abundance profiles. From clusters, 146 were identified by using MALDI-TOF/TOF mass spectrometry. Similarities as well strain-specific differences observed. production involved iron acquisition, pyocyanin biosynthesis, quinolone signalling nitrogen metabolism, proteases, oxidative general stress responses. Individual from classes highly represented both Strain-specific concerned within functional particularly for enzymes acquisition polysaccharide proteases. The results demonstrate isolate responds [Ca2+] fashion similar strain PAO1, but may allow this successfully colonize environment.