Corynebacterium glutamicum σ E is involved in responses to cell surface stresses and its activity is controlled by the anti-σ factor CseE

• DOI: 10.1099/mic.0.2007/012690-0
• Publication Date: 2008-02-29T00:43:38Z
• Authors: Soo-Dong Park; Jung-Won Youn; Young-Joon Kim; Seok-Myung Lee; Younhee Kim; Heung-Shick Lee
• Keywords: Corynebacterium glutamicum; FtsZ

In this study, we demonstrate that sigma(E), an alternative sigma factor of Corynebacterium glutamicum, is involved in cell surface stresses. Cells which the sigE gene was deleted evidenced increased sensitivity to magnesium deficiency, as well SDS, lysozymes, EDTA and heat. We utilized physiological analyses show downstream gene, designated cseE, encodes anti-sigma factor. The retarded growth cseE mutant cells under ordinary conditions could be recovered by additional deletion encoding sigma(E). Under stress conditions, phenotype cseE-overexpressing mimicked mutant. genes were transcribed into a single transcript, transcription stimulated SigE CseE proteins interacted physically vitro, form glutathione S-transferase (GST) maltose binding protein (MBP) fusion proteins, respectively. 2D-PAGE analysis wild-type crude extracts showed failed synthesize 34 kDa polypeptide normally induced grown heat (or SDS)-stressed conditions. turned out expressed from ORF NCgl1070 similarity methyltransferases may confer resistance antibiotics. Accordingly, extreme antibiotics, including nalidixic acid, penicillin vancomycin. Finally, present discussion possible role acclimation C. glutamicum