Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development

• DOI: 10.1099/vir.0.2008/003426-0
• Publication Date: 2008-11-13T19:43:46Z
• Authors: Ying Fang; Jane Christopher-...; Elizabeth Brown; Haixia Liu; Zhenhai Chen; Steven R. Lawson; Rachael Breen; Travis Clement; Xiaofei Gao; Jingjing Bao; David Knudsen; Russell Daly; Eric Nelson
• Keywords: Arterivirus; clone (Java method); Recombinant virus

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major problem in the pork industry worldwide. The limitations of current PRRSV vaccines require development new generation vaccines. One key steps future vaccine is include markers for diagnostic differentiation vaccinated animals from those naturally infected with wild-type virus. Using cDNA infectious clone type 1 PRRSV, this study constructed recombinant green fluorescent protein (GFP)-tagged containing deletion an immunogenic epitope, ES4, nsp2 region. In nursery pig disease model, was attenuated lower level viraemia comparison that parental To complement marker identification, GFP ES4 epitope-based ELISAs were developed. Pigs immunized lacked antibodies directed against corresponding deleted but generated high-level antibody response by 14 days post-infection. These results demonstrated virus, conjunction tests, enables serological between virus-infected This rationally designed will provide basis further assist control PRRS.