Characterization of liver histopathology in a transgenic mouse model expressing genotype 1a hepatitis C virus core and envelope proteins 1 and 2

• DOI: 10.1099/vir.0.80969-0
• Publication Date: 2005-07-20T23:27:56Z
• Authors: Turaya Naas; Masoud Ghorbani; Ikuri Alvarez-Maya; Michael Lapner; Rashmi Kothary; Yves De Repentigny; Susantha Gomes; Lorne Babiuk; Antonio Giulivi; Catalina Soare; Ali Azizi; Francisco Diaz-Mi...
• Keywords: Steatosis

Hepatitis C virus (HCV) is a major cause of chronic hepatitis and hepatocellular carcinoma worldwide. The purpose this study was to determine how the HCV structural proteins affect dynamic functional properties hepatocytes measure extra-hepatic manifestations induced by these viral proteins. A transgenic mouse model established expressing core, E1 E2 downstream CMV promoter. RNA detected using RT-PCR in tissues, such as liver, kidney, spleen heart. Expression transgene analysed real-time PCR quantify different tissues at ages. Immunofluorescence analysis revealed expression predominantly hepatocytes. Lower levels protein were kidneys. increased liver with age. Histological cells demonstrated steatosis mice older than 3 months, which more progressed Electron microscopy alterations nuclei, mitochondria endoplasmic reticulum. induce severe hepatopathy model. These became prone lymphoid tumour development carcinoma. In model, effects HCV, included swelling renal tubular cells, mild. It likely that mediate some histological interfering lipid transport metabolism.