Ebola virus glycoprotein GP is not cytotoxic when expressed constitutively at a moderate level

• DOI: 10.1099/vir.0.81361-0
• Publication Date: 2006-04-07T03:57:39Z
• Authors: Nathalie Alazard-...; Valentina Volchkova; Olivier Reynard; Caroline Carbonnelle; Olga Dolnik; Michèle Ottmann; Alexander Khromykh; Viktor E. Volchkov
• Keywords: P-glycoprotein; Ebolavirus

Transient expression of Ebola virus (EBOV) glycoprotein GP causes downregulation surface proteins, cell rounding and detachment, a phenomenon believed to play central role in the pathogenicity virus. In this study, evidence that moderate does not result such morphological changes was provided. It shown continuously produced 293T cells from Kunjin replicon correctly processed transported plasma membrane without affecting β 1 α 5 integrins major histocompatibility complex I molecules. The level GP-expressing similar observed infected with EBOV early infection lower than transfected plasmid DNA, phCMV-GP, expressing strong promoter. Importantly, transient transfection GP-coding DNA resulted overexpression GP, which lead molecules massive detachment cells. Here, it also demonstrated markers are late events infection, whereas synthesis release particles occur at steps do cause significant cytotoxic effects. These findings indicate virus-infected is controlled well by several mechanisms allow hence appearance its properties.