Infectivity of nanovirus DNAs: induction of disease by cloned genome components of Faba bean necrotic yellows virus

• DOI: 10.1099/vir.0.81753-0
• Publication Date: 2006-05-12T01:10:19Z
• Authors: T. Timchenko; L. Katul; M. Aronson; J. C. Vega-Arreguín; B. C. Ramirez; H. J. Vetten; B. Gronenborn
• Keywords: Acyrthosiphon pisum; Aphis craccivora; Infectivity

Circumstantial evidence suggests that the genome of Faba bean necrotic yellows virus (FBNYV), a nanovirus, consists eight distinct, circular, single-stranded DNAs, each about 1 kb and encoding only one protein. Here, use cloned full-length FBNYV DNAs for reproducing FBNYV-like symptoms in Vicia faba, principal natural host FBNYV, is reported. Characteristic infection were obtained faba plants following biolistic DNA delivery or agroinoculation with all DNAs. Although different have been invariably detected field samples infected various geographical isolates, experimental combinations fewer than also led to typical symptoms. Even five components, DNA-R, DNA-S, DNA-M, DNA-U1 DNA-U2, sufficient inducing disease V. upon agroinoculation. Symptomatic agroinoculated bombarded contained virions; however, was not transmitted by Aphis craccivora Acyrthosiphon pisum, two efficient aphid vectors FBNYV.