Estimating the temporal relationship between PrPSc detection and incubation period in experimental bovine spongiform encephalopathy of cattle

• DOI: 10.1099/vir.0.82987-0
• Publication Date: 2007-10-18T19:27:59Z
• Authors: M. E. Arnold; J. B. M. Ryan; T. Konold; M. M. Simmons; Y. I. Spencer; A. Wear; M. Chaplin; M. Stack; S. Czub; R. Mueller; P. R. Webb; A. Davis; J. Spiropoulos; J. Holdaway; S. A. C. Hawkins; A. R. Austin; G. A. H. Wells
• Keywords: Bovine spongiform encephalopathy; Incubation period

This study examines tissues from sequential-kill, time-course pathogenesis studies to refine estimates of the age at which disease-specific PrP (PrP(Sc)) can first be detected in central nervous system (CNS) and related peripheral ganglia cattle incubating bovine spongiform encephalopathy (BSE). Such are important for risk assessments these should removed slaughter prevent human animal exposure BSE infection. Tissues were examined dosed orally with 100 or 1 g BSE-infected brain. Incubation period data doses obtained attack rate sequential-kill studies. A statistical model, fitted by maximum likelihood, accounted differences lognormal incubation logistic probability infection between different dose groups. Initial detection PrP(Sc) during was invariably brainstem earliest 30 44 months post-exposure g- g-dosed groups, respectively. The point 50 % animals would immunohistochemistry applied medulla-obex estimated 9.6 1.7 before clinical onset cattle, respectively, a low any more than 12 onset. inconsistently dorsal root ganglia, concurrent after CNS, not all certain sympathetic ganglia.