TRACE: An unbiased method to permanently tag transiently activated inputs

• DOI: 10.1101/2020.02.02.927194
• Publication Date: 2020-02-04T02:45:21Z
• Authors: Nathalie Krauth; Valentina Khalil; Meet Jariwala; Noëmie Mermet-Joret; Anne-Katrine Vest...; Marco Capogna; Sadegh Nabavi
• Keywords: 0301 basic medicine; 03 medical and health sciences; viral tracing; innate fear; mouse behavior; Cellular Neuroscience; Neurosciences. Biological psychiatry. Neuropsychiatry; optogenetics; neural circuits; RC321-571

AbstractA fundamental interest in circuit analysis is to parse out the synaptic inputs underlying a behavioral experience. Toward this aim, we have devised an unbiased strategy that specifically labels the afferent inputs that are activated by a defined stimulus in an activity-dependent manner. We validated this strategy in four brain areas receiving known sensory inputs. This strategy, as demonstrated here, accurately identifies these inputs.