Interaction of RSC chromatin remodelling complex with nucleosomes is modulated by H3 K14 acetylation and H2B SUMOylationin vivo

• DOI: 10.1101/2020.03.02.972562
• Publication Date: 2020-03-03T13:15:53Z
• Authors: Neha Jain; Davide Tamborrini; Brian Evans; Shereen Chaudhry; Bryan J. Wilkins; Heinz Neumann
• Keywords: SWI/SNF; Histone code; Histone-modifying enzymes

Abstract Chromatin remodelling complexes are multi-subunit nucleosome translocases that reorganize chromatin in the context of DNA replication, repair and transcription. A key question is how these find their target sites on chromatin. Here, we use genetically encoded photo-crosslinker amino acids to map footprint Sth1, catalytic subunit RSC (remodels structure chromatin) complex, living yeast. We interaction Sth1 bromodomain with H3 tail depends K14 acetylation by Gcn5. This modification does not recruit but mediates its neighbouring nucleosomes. observe a preference for H2B SUMOylated nucleosomes vivo show this moderately enhances binding vitro . Furthermore, ejected from mitosis, mode differs between interphase mitosis. In sum, our analyses recruitment specific targets involves multiple histone modifications most likely combination other components such as variants transcription factors. Key Points photo-crosslinking reveals ATPase nucleosome. binds K14ac via C-terminal ATPase-subunit Sth1. preferentially localizes H2B-SUMOylated