Molecular Basis of Sex Difference in Neuroprotection induced by Hypoxia Preconditioning in Zebrafish

• DOI: 10.1101/2020.06.10.144097
• Publication Date: 2020-06-11T04:32:03Z
• Authors: Tapatee Das; Kalyani Soren; Mounica Yerasi; Avijeet Kamle; Arvind Kumar; Sumana Chakravarty
• Keywords: Hypoxia; Stroke; Ischemic Preconditioning

ABSTRACT Hypoxia, the major cause of ischemia, leads to debilitating disease in infants via birth asphyxia and cerebral palsy, whereas adults heart attack stroke. A widespread, natural protective phenomenon termed ‘Hypoxic Preconditioning’ occurs when prior exposures hypoxia eventually results robust resistance. Accordingly, we have developed a novel model sex-specific hypoxic preconditioning adult zebrafish mimic tolerance mini stroke(s) human, which appears protect against severe damage inflicted by stroke event. Remarkable difference progression pattern neuroprotection between followed acute (PH) group, (AH) group were observed with noticeable sex difference. Since gender has been reported stroke, it was pertinent investigate whether any such also exists PH’s mechanism ischemic In order elucidate neural molecular mechanisms behind induced PH, high throughput proteomics approach utilizing iTRAQ performed, protein enrichment analysis using Ingenuity Pathway Analysis. Out thousands altered proteins brain, ones having critical role either neuroglial proliferation/differentiation or neurotrophic functions, validated analyzing their expression levels AH normoxia groups. Results indicate that female brains are more protected severity AH. The study sheds light on involvement many signaling pathways underlying pre-conditioning neuroprotective mechanism, can be further for therapeutic approach.