Multi-epitope Based Peptide Vaccine Design Using Three Structural Proteins (S, E, and M) of SARS-CoV-2: AnIn SilicoApproach

• DOI: 10.1101/2020.06.13.149880
• Publication Date: 2020-06-14T05:05:17Z
• Authors: Arpita Singha Roy; Mahafujul Islam Q...; Atqiya Fariha; Ithmam Hami; Ibrahim Khalil Afif; Md. Adnan Munim; Mohammad Rahanur ...; Md. Shahadat Hossain
• Keywords: Antigenicity; Peptide vaccine; Coronavirus; Epitope mapping; Docking (animal)

Abstract Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is the novel coronavirus responsible for ongoing pandemic of disease (COVID-19). No sustainable treatment option available so far to tackle such a public health threat. Therefore, designing suitable vaccine overcome this hurdle asks immediate attention. In study, we targeted design multi-epitope based using immunoinformatics tools. We considered structural proteins S, E and, M SARS-CoV-2, since they facilitate infection virus into host cell and different bioinformatics tools servers, predicted multiple B-cell T-cell epitopes having potential required design. Phylogenetic analysis provided insight on ancestral molecular changes evolutionary relationship E, proteins. Based antigenicity surface accessibility these proteins, eight were selected by various B T epitope prediction Molecular docking was executed interpret binding interactions three WTAGAAAYY, YVYSRVKNL, GTITVEELK their noticeable higher affinity scores −9.1, −7.4, −7.0 kcal/mol, respectively. Targeted had 91.09% population coverage worldwide. summary, identified most significant properties peptide-based against SARS-CoV-2.