The identity of human tissue-emigrant CD8+T cells

• DOI: 10.1101/2020.08.11.236372
• Publication Date: 2020-08-11T23:52:01Z
• Authors: Marcus Buggert; Laura A. Vella; Son Nguyen; Vincent Wu; Takuya Sekine; André Perez-Potti; Colby R. Maldini; Sasikanth Manne; Samuel Darko; Amy Ransier; Leticia Kuri-Cerv...; Alberto Sada Japp; Irene Bukh Brody; Martin A. Ivarsson; Laura Hertwig; Jack P. Antel; Matthew E. Johnson; Afam Okoye; Louis Picker; Golnaz Vahedi; Ernesto Sparrelid; Sian Llewellyn-Lacey; Emma Gostick; Niklas Björkström; Amit Bar-Or; Yoav Dori; Ali Naji; David H. Canaday; Terri M. Laufer; Andrew D. Wells; David A. Price; Ian Frank; Daniel C. Douek; E. John Wherry; Maxim G. Itkin; Michael R. Betts
• Keywords: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

ABSTRACT Lymphocyte migration is essential for human adaptive immune surveillance. However, our current understanding of this process rudimentary, because most studies to date have been restricted immunological analyses blood and various tissues. To address issue, we used an integrated approach characterize tissue-emigrant cells in thoracic duct lymph (TDL). In humans non-human primates, lymphocytes were by far the abundant lineage population efferent lymph, a vast majority these T cells. Cytolytic CD8 + cell subsets clonotypically discrete selectively confined intravascular circulation, persisting months after inhibition S1P-dependent tissue egress FTY-720. contrast, non-cytolytic with stem-like epigenetic transcriptional signatures predominated tissues TDL. Collectively, data provide atlas migratory system define nature that recirculate via