Dual RNA-Seq analysis of SARS-CoV-2 correlates specific human transcriptional response pathways directly to viral expression

• DOI: 10.1101/2021.02.09.430517
• Publication Date: 2021-02-10T04:52:31Z
• Authors: Nathan D Maulding; Spencer Seiler; Alex Pearson; Nick Kreusser; Josh Stuart
• Keywords: Pandemic; RNA-Seq

Abstract The SARS-CoV-2 pandemic has challenged humankind’s ability to quickly determine the cascade of health effects caused by a novel infection. Even with unprecedented speed at which vaccines were developed and introduced into society, identifying therapeutic interventions drug targets for patients infected virus remains important as new strains may evolve, or future coronaviruses emerge, that are resistant current vaccines. application transcriptomic RNA sequencing samples shed light on pathways involved in viral mechanisms host responses. We describe “dual RNA-seq” analysis consider both pathogen transcriptomes simultaneously, investigate first time co -regulation human genes. Together differential expression analysis, we tissue specificity expression, an inferred lipopolysaccharide response, co-regulation CXCL’s, SPRR’s, S100’s expression. Lipopolysaccharide response particular offer promise research prospect subgrouping based chemokine help explain vastly different reactions have Taken together these findings illuminate previously unappreciated signatures, identify considerations, contribute pipeline studying multi-transcriptome systems.