Blood flow coordinates collective endothelial cell migration during vascular plexus formation and promotes angiogenic sprout regression via vegfr3/flt4

• DOI: 10.1101/2021.07.23.453496
• Publication Date: 2021-07-25T06:30:10Z
• Authors: Yan Chen; Zhen Jiang; Katherine H. Fisher; Hyejeong R. Kim; Paul C. Evans; Robert N. Wilkinson
• Keywords: Venous plexus; Plexus; Arteriogenesis

Abstract Nascent vascular networks adapt to the increasing metabolic demands of growing tissues by expanding via angiogenesis. As expand, blood vessels remodel, progressively refining connectivity generate a more haemodynamically efficient network. This process is driven interplay between endothelial cell (EC) signalling and flow. While much known about angiogenesis, considerably less understood mechanisms underlying vessel remodelling Here we employ zebrafish sub-intestinal venous plexus (SIVP) characterise flow-dependent remodelling. Using live imaging track ECs show that flow controls SIVP coordinating collective migration within developing plexus. Blood opposes continuous ventral EC required for regression angiogenic sprouts support growth. Sprout occurs coordinated polarisation from non-perfused leading sprouts, which migrate in opposition incorporate into SIV. compatible with low dependent upon vegfr3/flt4 function under these conditions. limits expansive promoted . Collectively, studies reveal how sculpts balancing