Phosphate transporter PHT1;1 as a key determinant of phosphorus acquisition in Arabidopsis natural accessions

• DOI: 10.1101/2022.01.05.475091
• Publication Date: 2022-01-06T18:25:20Z
• Authors: Pei-Shan Chien; Ya-Ting Chao; Chia-Hui Chou; Yu-Ying Hsu; Su-Fen Chiang; Chih-Wei Tung; Tzyy-Jen Chiou
• Keywords: Genome-wide Association Study

ABSTRACT To understand the genetic basis in governing phosphorus (P) acquisition, we performed genome-wide association studies (GWAS) on a diversity panel of Arabidopsis thaliana by two primary determinants P phosphate (Pi)-uptake activity and PHOSPHATE TRANSPORTER 1 (PHT1) protein abundance. Association mapping revealed shared significant peak chromosome 5 (Chr5) where PHT1;1 / 2 3 genes reside, suggesting strong correlation between regulation Pi-uptake PHT1 Genes encoding transcription factors, kinases, metalloprotease associated with both traits were also identified. Conditional GWAS followed statistical analysis genotype-dependent expression assays an epistatic interaction MYB DOMAIN PROTEIN 52 ( MYB52 ) Chr1. Analyses F1 hybrids generated crossing subgroups natural accessions carrying specific SNPs further effects potential variants Pi uptake activity. Notably, soil contents A. habitats found to coincide haplotype, underscoring how fine-tuning acquisition allows plants adapt their environments. This study sheds light complex offers framework systematically assess effectiveness approaches quantitative traits. One sentence summary Stepwise analyses reveal insights into regulating associations transporter haplotype habitats.