The Impact of Storage Condition and Duration on Function of Native and Cargo-Loaded Mesenchymal Stromal Cell Extracellular Vesicles

• DOI: 10.1101/2022.06.14.496108
• Publication Date: 2022-06-17T09:55:20Z
• Authors: Daniel Levy; Anjana Jeyaram; Louis J. Born; Kai-Hua Chang; Sanaz Nourmohamma...; Angela Ting Wei Hsu; Talia Solomon; Amaya Aranda; Samantha Stewart; Xiaoming He; John W. Harmon; Steven M. Jay
• Keywords: Extracellular vesicles; Exosome; Extracellular Vesicles

Abstract As evidenced by ongoing clinical trials and increased activity in the commercial sector, extracellular vesicle (EV)-based therapies have begun transition from bench to bedside. this progression continues, one critical aspect EV translation is understanding effects of storage transport conditions. Several studies assessed impact on characteristics such as morphology, uptake, component content, but duration temperature functional bioactivity and, especially, loaded cargo are scarcely reported. Here, outcomes following at different temperatures (room temperature, 4°C, -20°C, -80°C) for various durations well after lyophilization were assessed. Mesenchymal stem/stromal cell (MSC) EVs observed retain key aspects their (pro-vascularization, anti-inflammation) up 4-6 weeks -80°C, lyophilization. Furthermore, via vitro assays an vivo wound healing model respectively, these same conditions also demonstrated enable preservation functionality microRNA (miRNA) long non-coding RNA (lncRNA) MSC EVs. These findings extend current how therapeutic potential impacted may inform best practices handling both basic research translational purposes.