Spatial cell type mapping of multiple sclerosis lesions

• DOI: 10.1101/2022.11.03.514906
• Publication Date: 2022-11-04T03:20:14Z
• Authors: Celia Lerma-Martin; Pau Badia-i-Mompel; Ricardo O. Ramire...; Patricia Sekol; Annika Hofmann; Thomas Thäwel; Christian J. Riedl; Florian Wünnemann; Miguel A. Ibarra-...; Tim Trobisch; Philipp Eisele; Denis Schapiro; Maximilian Haeussler; Simon Hametner; Julio Saez-Rodriguez; Lucas Schirmer
• Keywords: Cell type

Abstract Multiple sclerosis (MS) is a prototypic chronic-inflammatory disease of the central nervous system. After initial lesion formation during active demyelination, inflammation gradually compartmentalized and restricted to specific tissue areas such as rim in chronic-active lesions. However, cell type-specific spatially drivers chronic damage expansion are not well understood. Here, we investigated properties subcortical white matter lesions by creating spatial map gene expression across various inflammatory stages MS. An integrated analysis single-nucleus transcriptomics data enabled us uncover patterns glial, immune stromal subtype diversity, identify cell-cell communication signaling signatures non-lesion Our results provide insights into conversion microenvironment from ‘homeostatic’ pathogenic or ‘dysfunctional’ state underlying progression We expect that this study will help resolved biomarkers therapeutic targets for future interventional trials