Risk factors for extended-spectrum beta-lactamase (ESBL) producingE. colicarriage among children in a food animal producing region of Quito, Ecuador

• DOI: 10.1101/2022.11.14.22282279
• Publication Date: 2022-11-15T03:15:11Z
• Authors: Heather K. Amato; Fernanda Loayza; Liseth Salinas; Diana Paredes; Daniela Garcia; Soledad Sarzosa; Carlos Saraiva-Ga...; Timothy J. Johnson; Amy J. Pickering; Lee W. Riley; Gabriel Trueba; Jay P. Graham
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences; 13. Climate action; 15. Life on land; 3. Good health

AbstractBackgroundThe spread of antibiotic-resistant bacteria may be driven by human-animal-environment interactions, especially in regions with limited restrictions on antibiotic use, widespread food animal production, and free-roaming domestic animals. In this study, we aimed to identify risk factors related to domestic animals, backyard food animals, and commercial food animal production in Ecuadorian communities.Methods & FindingsWe conducted a repeated-measures study from 2018-2021 in 7 semi-rural parishes of Quito, Ecuador to identify determinants of third-generation cephalosporin-resistantE. coli(3GCR-EC) and extended-spectrum beta-lactamaseE. coli(ESBL-EC) in children and domestic animals. We used multivariable log-binomial regression models to estimate relative risks (RR) of 3GCR-EC and ESBL-EC carriage. We collected 1,699 child fecal samples from 600 households and 1,871 animal fecal samples from 376 of the same households. Risk factors for 3GCR-EC included living within 5 km of more than 5 commercial food animal operations (RR: 1.36; 95% Confidence Interval: 1.16, 1.59), household pig ownership (1.23; 1.02, 1.48), child pet contact (1.23; 1.09, 1.39), and rarely/never washing hands after contact with animals (1.15; 0.98, 1.34). Risk factors for ESBL-EC were dog ownership (1.43; 1.00, 2.04), child pet contact (1.54; 1.10, 2.16), placing animal feces on household land/crops (1.63; 1.09, 2.46), and combined exposures to both household food animals and commercial food animal operation drainage paths (1.80; 0.94, 3.45).ConclusionsPolicies and interventions that improve the safety of animal waste management in communities and in commercial food animal production operations may be necessary to curb the spread of resistant bacteria.