Emergence of ISAba1-linked oxacillinase genes among carbapenem resistantAcinetobacter baumanniiisolates in a tertiary cardiac center, Nepal

• DOI: 10.1101/2023.03.13.532405
• Publication Date: 2023-03-14T05:30:10Z
• Authors: Shrijana Bista; Bindeshwar Yadav; Gopiram Syangtan; Jivan Shakya; Reshma Tuladhar; Dev Raj Joshi; Binod Lekhak
• Keywords: Acinetobacter baumannii; Agar dilution; Carbapenem

Abstract Insertion sequence contributes to the emergence of carbapenem resistance by dissemination carbapenemase genes and providing promoter for their overexpression. This study aims ascertain occurrence IS Aba1 -linked OXA its relevance level in Acinetobacter baumannii . hospital based descriptive was conducted at Shahid Gangalal National Heart Center, Kathmandu, Nepal. An overall 1,291 clinical specimens received routine culture antibiotic susceptibility testing throughout period were included this study. Identification validated through detection intrinsic bla OXA-51-like gene polymerase chain reaction (PCR). Antibiotic tested Kirby-Bauer disc diffusion approach minimum inhibitory concentration (MIC) meropenem assessed agar dilution method. Uniplex PCR assays performed detect encoding oxacillinases Upstream association insertion element, oxacillinase mapping strategy using F OXA-51R/OXA-23R primers. Out 340 bacteria isolated, only 40 (11.8%) All isolates resistant against with MIC value ranging from 16-256 μg/ml. OXA-23-like present every isolate but OXA-58 just two (5%). had either above or gene. Higher 90 (243.20 μg/ml) found A cluster upstream both genes, thus depicting eminent role enhanced resistance. are rapidly emerging settings Thus, medical communities need be prepared enable targeted approaches managing burgeoning problem