Exposure to titanium dioxide nanoparticles accelerates abnormal fat deposition through the mediation of the ROS via the AGE-RAGE signaling pathway in mice

• DOI: 10.1101/2023.08.24.554746
• Publication Date: 2023-08-25T20:30:17Z
• Authors: Jingyi Liu; Liang Zhong; Tiantian Yuan; Jingchun Sun; Yaxin Wang; Jiahao Chen; Gongshe Yang; Taiyong Yu
• Keywords: RAGE; Lipid droplet; Titanium Dioxide

Abstract Titanium dioxide nanoparticles (TiO 2 NPs) are widely added to various types of foods as food additives. Previous studies have shown that TiO NPs exposure can cause abnormal deposition adipose tissue in organisms, resulting lipid metabolism disorder. However, the potential molecular mechanisms underlying NPs’ effects yet be elucidated. In this study, our data indicated (100 mg/Kg BW, 20 nm) accelerated fat epididymal tissues and disturbed level blood glucose subsequently normal-fat diet mice. Further showed at a concentration 100 µg/mL significantly induced proliferation differentiation 3T3-L1 preadipocytes. Mechanistic we revealed overproduction reactive oxygen species (ROS) cytoplasm, which upregulated expression receptors for advanced glycation end products (AGEs) leading accumulation adipocytes with higher ROS. antioxidant N-acetylcysteine (NAC) was therapeutic overaccumulation adipocytes. This study provides insight into mechanism by highlighted need reevaluation food-grade daily life.