MCAK Recognizes the Nucleotide-Dependent Feature at Growing Microtubule Ends

• DOI: 10.1101/2023.10.02.560461
• Publication Date: 2023-10-03T03:45:16Z
• Authors: Wei Chen; Yin-Long Song; Jian-Feng He; Xin Liang
• Keywords: Kinesin

Abstract The growing plus-end is a key regulatory site for microtubule dynamics. MCAK (mitotic centromere-associated kinesin), depolymerizing kinesin, an end-binding regulator of catastrophe frequency. It intriguing how specifically binds to dynamic ends. Here, we measure the kinetics using single-molecule imaging and reveal preference. entire GTP cap, including EB cap distalmost cap. Further analysis shows that strongly GTPγS microtubules, suggesting it could recognize nucleotide-dependent feature microtubules. Moreover, binding preference independent on nucleotide state MCAK, this facilitates high-affinity MCAK. Finally, show despite partially sharing regions, XMAP215 function in additive manner, demonstrating simple logic regulators work co-ordination. In all, our results provide novel insights into understanding regulates dynamics