Woollymutation withGet02locus overcomes the polygenic nature of trichome-based pest resistance in tomato

• DOI: 10.1101/2023.10.06.561305
• Publication Date: 2023-10-13T14:00:13Z
• Authors: Eloisa Vendemiatti; Inty O. Hernández...; Roxane Snijders; Tanmayee Torne; Rodrigo Therezan; Gabriela Prants; Carlos Lopez-Ortiz; Umesh Reddy; Petra Bleeker; Craig A. Schenck; Lázaro Eustáquio ...; Vagner Augusto Be...
• Keywords: Trichome; Wild type

ABSTRACT Type-IV glandular trichomes, which only occur in the juvenile phase of tomato development, produce acylsugars (AS) that broadly protect against arthropod herbivory. Previously, we introgressed capacity to retain type-IV trichomes adult from Solanum galapagense into cv. Micro-Tom (MT). The resulting MT- Get line contained five loci associated with enhancing density plants. We genetically dissected and obtained a sub-line containing locus on chromosome 2 (MT- Get02 ). This genotype displayed about half compared wild progenitor. However, when stacked gain-of-function allele WOOLLY , codes for HD-ZIP IV transcription factor, Get02/Wo exhibited double number S. . discovery corroborates previous reports positioning as master regulator trichome development. AS levels were comparable progenitor, although composition types differed, especially regarding less medium-length acyl chains. Agronomical parameters including yield, MT. Pest resistance assays showed enhanced protection whitefly, caterpillar, fungus Septoria lycopersici did not reach suggesting specificity particulars pest mechanism. Our findings trichome-mediated advance development robust, naturally resistant varieties, harnessing potential natural genetic variation. Moreover, by manipulating two loci, achieved exceptional results highly complex, polygenic trait, such herbivory tomato.