ChAHP2 and ChAHP control diverse retrotransposons by complementary activities

• DOI: 10.1101/2024.02.05.578923
• Publication Date: 2024-02-07T16:30:29Z
• Authors: Josip Ahel; Aparna Pandey; Michaela Schwaiger; Fabio Mohn; Anja Basters; Georg Kempf; Aude Andriollo; Lucas Kaaij; Daniel Hess; Marc Bühler
• Keywords: Retrotransposon; Transcription

ABSTRACT Retrotransposon control in mammals is an intricate process that effectuated by a broad network of chromatin regulatory pathways. We previously discovered ChAHP, protein complex with repressive activity against SINE retrotransposons, composed the transcription factor ADNP, remodeler CHD4, and HP1 proteins. Here we identify ChAHP2, homologous to wherein ADNP replaced ADNP2. ChAHP2 predominantly targeted ERVs LINEs, via HP1β-mediated binding H3K9 trimethylated histones. further demonstrate ChAHP also binds these elements mechanistically equivalent manner distinct from DNA sequence-specific recruitment at SINEs. Genetic ablation ADNP2 alleviates ERV LINE1 repression, which synthetically exacerbated additional depletion ADNP. Together, our results reveal complexes function both non-autonomous autonomous retrotransposons complementary activities, adding complexity mammalian transposon control.