Agrobacterium-mediatedCuscuta campestristransformation as a tool for understanding plant-plant interactions

• DOI: 10.1101/2024.02.23.581736
• Publication Date: 2024-02-27T17:51:45Z
• Authors: Supral Adhikari; Asha Mudalige; Lydia Phillips; Hyeyoung Lee; Vivian Bernal-Gal...; Hope Gruszewski; James H. Westwood; So-Yon Park
• Keywords: Cuscuta; Parasitic plant

Summary Cuscuta campestris , a stem parasitic plant, has served as valuable model plant for the exploration of plant-plant interactions and molecular trafficking. However, major barrier to C. research is that method generate stable transgenic plants not yet been developed. Here, we describe development transformation protocol using various reporter genes (GFP, GUS, or RUBY) morphogenic ( CcWUS2 CcGRF/GIF ), ultimately leading robust Agrobacterium -mediated transformation. The stably transformed regenerated RUBY produced haustoria, signature organ plants, these were functional in forming host attachments. locations T-DNA integration parasite genome confirmed through TAIL-PCR. Transformed also flowers seeds exhibiting betalain pigment, providing proof germline transmission transgene. Furthermore, only useful selectable marker transformation, but provides insight into movement molecules from during parasitism. Thus, reported here overcomes obstacle opens new possibilities studying their with hosts.