The DivisionCounter, a method for counting large ranges of cell divisionsin vivo,reveals cell dynamics of leukemic cell killing via CAR-T therapy

• DOI: 10.1101/2024.06.24.600543
• Publication Date: 2024-06-29T00:30:19Z
• Authors: Lucie S P Hustin; Cecile Conrad; Chang Liu; Jaime Fuentealba; Silvia Menegatti; Seva Shneer; Aude Battistella; Fanny Tabarin; Tom S Weber; Sebastian Amigorena; Ken R Duffy; Leïla Perié
• Keywords: Multicellular organism

Abstract Cell division drives multicellular growth and its dysregulation can cause disease. While approximately 44 divisions are needed to produce without death all 10 13 cells in the human body, current methods limited count cell vivo across diverse mammalian types. Here we introduce DivisionCounter, a method over large ranges (∼70 divisions) using an easy fluorescence readout. We demonstrate that DivisionCounter accurately measures average of both vitro . Its use revealed leukemia tumor rates independent organ’s specific microenvironment CAR-T treatment, providing estimate The holds unique potential for quantifying contributions division, death, migration healthy pathological tissues.